Genetic Polymorphisms of CYP2C19 Enzyme in Donors and Recipients after Liver Transplantation

P Badiee, Z Hashemizadeh, S A Malek-Hosseini, B Geramizadeh

Abstract


Background: Liver transplant recipients are treated with various drugs, the metabolism of which is dependent on the cytochrome P450 polymorphic genotype.

Objective: To identify the polymorphic variety of CYP2C19 genotype in liver allograft before and after transplantation.

Methods: The study was conducted on 88 liver recipients. The CYP2C19 genotypes in donors and recipients were the same in 32 and different in 56 recipients. Extracted genomic DNA from the leukocytes and liver graft tissues were analyzed by TaqMan SNP genotyping assay. The distributions of homozygote, heterozygote, poor and ultra-rapid metabolizers’ genotypes were investigated in both groups.

Results: The distributions of CYP2C19 genotypes before transplantation in the blood and liver graft were within the normal range. After transplantation, in patients with different CYP2C19 genotype in donors and recipients, the genotypes of homozygote and ultra-rapid metabolizers were significantly decreased (p=0.024); the heterozygotes and poor metabolizer genotypes were significantly increased (p=0.017).

Conclusion: The variety in CYP2C19 genotyping must be considered in patients with different genotypes in donor and recipients to predict the dosage regimens, optimize the treatment and decrease toxicity.


Keywords


Cytochrome P450; Poor metabolizer; CYP2C19; Genotype; Ultra-rapid metabolizer; Liver transplantation

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 pISSN: 2008-6489
 eISSN: 2008-6490