Urinary CXCL9 and CXCL10 Levels and Acute Renal Graft Rejection

H Senturk Ciftci, T Tefik, M K Savran, E Demir, Y Caliskan, Y D Ogret, T Oktar, O Sanlı, T Kocak, Y Ozluk, F S Oguz, I Kilicaslan, F Aydın, A Turkmen, I Nane

Abstract


Background: Monitoring of chemokines, CXCL9 and CXCL10, in serum may present a non-invasive detection method for rejection.

Objective: To investigate the relationship between urinary levels of CXCL9 and CXCL10 and graft function following renal transplantation.

Methods: 75 living-related donor renal transplant recipients were studied. Urinary levels of chemokines were collected pre-operatively, on post-operative 1st day, 7th day, 1st month, 3rd month, and at the time of rejection. Chemokines levels were assayed using and enzyme-linked immunosorbent assay.

Results: Clinical variables were monitored. 10 (15%) patients had biopsy-proven rejection during the follow-up period. The urinary CXCL9 level in those with rejection was significantly higher than that in those with non-rejection group at the 1st day (p<0.001), 7th day (p<0.001), and at the time of rejection (p=0.002). The urinary CXCL10 level was also significantly higher in those with rejection compared with non-rejection group at 1st day (p<0.001), 7th day (p<0.001), and at the time of rejection (p=0.001). Serum creatinine level was strongly correlated with the urinary CXCL9 and CXCL10 levels at the time of rejection (r=0.615, p=0.002; and r=0.519, p=0.022, respectively). Among those with T cell-mediated rejections the mean urinary CXCL10 level increased to as high as 258.12 ng/mL.

Conclusion: Urinary CXCL9 and CXCL10 levels might have a predictive value for T cell-mediated rejection in early post-transplantation period. Measurement of urinary CXCL9 and CXCL10 levels could provide an additional tool for the diagnosis of rejection.


Keywords


Chemokines; Renal transplantation; Rejection; Biomarker; Graft function

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 pISSN: 2008-6489
 eISSN: 2008-6490