Endothelial Nitric Oxide Synthase Gene T-786C Polymorphism in Renal Transplant Recipients

N Azarpira, B Geramizadeh, S Nikeghbalian, A Bahador, R Yaghobi, H Karimi, M Ayatolahi, M Aghdai, H Salahi, SA Malek-Hosseini, J Roozbeh, M Sagheb, GA Raisjalali, A Behzadi


Background: Nitric oxide (NO) is a major mediator in vascular biology, regulating regional blood flow. NO and the enzymes required for its production contribute to ischemia-reperfusion injury. The T-786C functional polymorphism in the promoter region substantially reduces promoter activity of the endothelial nitric oxide synthase (eNOS) gene and compromises endothelial NO synthesis.

Objective: To examine the association between T-786C (rs 2070744) single nucleotide polymorphism (SNP) in eNOS gene and the development of acute rejection in renal transplant patients.

Methods: 60 renal transplant recipients (30 with episodes of acute rejection (ARs) and 30 without rejection (non-ARs)), between June 2008 and March 2010, were included in this study. The polymorphism was determined by PCR-restriction fragment-length polymorphism analysis.

Results: The distribution of the genotypes were TT/TC/CC 60%, 33.4%, 6.6%, and 43%, 46.7%, 13.3% in ARs and non-ARs, respectively (p=0.28). The frequency of T-allele was 76.7% and 66.3%; and for C-allele was 66.6% and 33.3% in ARs and non-ARs, respectively (p=0.09). There were no significant associations between these polymorphisms and acute and chronic kidney allograft rejection.

Conclusion: We could not detect any significant association between polymorphism in T-786C of eNOS gene and the development of acute rejection.


Nitric oxide; Endothelial nitric oxide synthase; Polymorphism; Transplant; Acute rejection

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 pISSN: 2008-6482
 eISSN: 2008-6490


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